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OBJECTIVE: To investigate the antibacterial agents in the marine fungus Penicillium sp. F00120 from the deep sea sediments in the South China Sea. METHODS: The compounds were purified by a combination of chromatographic methods, and their structures were identified by MS, 1H-NMR, and 13C-NMR spectroscopic analysis. The antimicrobial activities of the isolates were evaluated by the agar plate diffusion method. RESULTS: Eight compounds were isolated from the culture and were characterized as ergosta-4, 6, 8(14), 22-tetraen -3-one(1), 25-hydroxyergosta4, 6, 8(14), 22-tetraen-3-one(2), ergosta-7, 22-dien-3β, 5α, 6β-triol (3), ergosta-5, 7, 22-trien-3β-ol(4), 5α, 8α-epidioxyergosta-6, 22-dien-3β-ol(5), 5α, 6α-epoxyergost-8(14), 22-dien-3β, 7α-diol(6), cholesterol(7), and 4-hydroxyacetophenone(8), respectively. CONCLUSION: Compounds 1-3 and 6-8 are isolated from this fungus for the first time. Compounds 3, 4, and 8 shows moderate antibacterial activities against P. aeruginosa, S. aureus, and E. coll.
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Objective: To study the flavonoids from the roots of Ephedra sinica in Ephedra Tourn. ex L. Methods: The roots of E. sinica were extracted with 90% ethanol, and isolated and purified by silica gel, Sephadex LH-20, and RP-18 gel column chromatography. All the compounds were identified based on spectral analyses (including MS, 1H-NMR, and 13C-NMR). Results: Thirteen flavonoids were isolated from the roots of E. sinica and were characterized as ephedrannin A (1), mahuannin A (2), mahuannin B (3), mahuannin D (4), apigenin (5), kaempferol (6), quercetin (7), dihydroquercetin (8), 3′, 4′, 5, 7-tetrahydroxy flavanone (9), (+)-catechin (10), (-)-epi-catechin (11), afzelechin (12), and (-)-epi-afzelechin (13), respectively. Conclusion: Compounds 7-13 are obtained from this plant for the first time.
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Objective: To study the chemical constituents from the roots of Asparagus filicinus. Methods: Silica gel, Sephadex LH-20, ODS column chromatography, and preparative TLC were used to isolate and purify the compounds. All the compounds were identified based on spectral analysis (including MS, 1H-NMR, and 13C-NMR). Results: Sixteen phenolic compounds were isolated from 90% ethanol extract in the roots of A. filicinus and were characterized as 3′-methoxynyasin (1), (+)-nyasol (2), (-)-4′-O-methyl-nyasol (3), iso-agatharesinol (4), gobicusin A (5), 4-[5-(4-hydroxyphenoxy)-3-penten-1-ynyl] phenol (6), 1-methoxy-2-hydroxy-4-[5-(4-hydroxyphenoxy)-3-penten-1-ynyl] phenol (7), gobicusin B (8), 1-O-p-coumaroyl-3-O-feruloylglycerol (9), 1, 3-di-O-feruloylglycerol (10), syringic acid (11), 4-hydroxybenzoic acid (12), fumalic acid (13), caffeic acid (14), trans-coniferyl alcohol (15), and vanillic acid (16), respectively. Conclusion: These phenolic compounds are isolated from A. filicinus for the first time except compounds 2 and 12.
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<p><b>OBJECTIVE</b>To develop an HPLC-ELSD method for determination of vetatramine. in Veratrum nigrum.</p><p><b>METHOD</b>The analy fical column was Shim-pack ODS - C18 (4.6 mm x 250 mm, 4 microm) column, the mobile phase was acetonitrile-water (containing 0.1% triethylamine) (50:50), at a flow rate of 0.8 mL x min(-1). The temperature of drift tube was 90 degrees C and the gas flow was at the rate of 2.5 L x min(-1).</p><p><b>RESULT</b>The calibration curve was linear in the range of 0.36-3.6 microg (r = 0.999 8). The average recovery was 100.9% (RSD 2.3%, n = 6). The contents of veratramine in Veratrum nigrum. from the ten different sources were determined.</p><p><b>CONCLUSION</b>The method may be used as a accurate and reproducible way to determine the content of veratramine in V. nigrum.</p>
Subject(s)
Chromatography, High Pressure Liquid , Drugs, Chinese Herbal , Chemistry , Linear Models , Reproducibility of Results , Sensitivity and Specificity , Veratrum , Chemistry , Veratrum AlkaloidsABSTRACT
<p><b>OBJECTIVE</b>To investigate the preparation technique and optimal formulation of Fuyankang dispersed tablets.</p><p><b>METHOD</b>The formula of Fuyankang dispersed tablets were optimized in terms of disintegrating time by studying single factor and orthogonal design test.</p><p><b>RESULT</b>The products formulated with the optimum techniques met the quality specification of dispersed tablets. The dissolubility of the optimized dispersed tables was obviously faster than that of common tablets.</p><p><b>CONCLUSION</b>This prescription and technology of Fuyankang dispersed tablets are reasonable and effective.</p>